English
聯系我們
網站地圖
郵箱
舊版回顧



城市環境所利用單細胞拉曼光譜研究土壤解磷微生物取得進展

文章來源:城市環境研究所   發布時間:2019-05-10  【字號:     】  

  磷是一種至關重要但不可再生的資源。磷肥是作物生長必須三大營養元素(氮、磷、鉀)之一,但磷礦是有限資源,據估計,以目前的磷礦儲備,大概還能繼續開采100年,因此,磷又被稱為“正在消失的元素”。隨著長期大量的磷肥施用,土壤中積累了大量固定態磷,大大降低了磷素的生物有效性,加劇了磷資源短缺的問題,對土壤固定態磷的活化成為未來發展可持續農業和磷肥利用的重要戰略。

  微生物是土壤元素循環的發動機,解磷微生物可以通過釋放有機酸溶解土壤中的鈣磷,或釋放活性磷酸酶活化土壤中的有機磷。利用解磷微生物釋放土壤中被固定的磷,提升磷的生物有效性,對磷素的可持續利用和緩解磷礦枯竭危機具有至關重要的作用。然而,環境微生物的大量不可純培養性,對開發利用微生物資源帶來了很大的挑戰。從單細胞水平上研究環境微生物可克服純培養或富集培養的限制,實現在環境介質下的原位研究,拉曼光譜可在單細胞水平上對微生物進行無損檢測。

  中國科學院城市環境研究所朱永官研究團隊發展了利用單細胞拉曼結合重水標記篩選高活性土壤解磷微生物的新方法。該方法可以無損檢測土壤環境中不可培養的微生物,從而挖掘出具有強解磷功能的微生物。方法原理是:在僅固定態無機磷(如難溶磷酸三鈣)或有機磷(如卵磷脂)存在的環境中,具有解磷功能的微生物由于仍具活性,甚至生長繁殖能力,因此能同化重水中的氘,產生特征的C-D拉曼峰。解磷微生物與非解磷微生物在C-D拉曼峰存在巨大的強度差異,解磷能力越強的微生物具有越強的C-D拉曼峰,利用這一特性可以靈敏快速地篩選出土壤中的解磷微生物。重水標記由于不改變環境營養底物庫,可更真實反映土壤環境中的解磷微生物。上述工作促進了對未知環境菌群的深入認識,尤其是磷循環功能菌及其活性的深入解析。研究成果以D2O Isotope Labeling Approach to Probing Phosphate Solubilizing Bacteria in Complex Soil Communities by Single-Cell Raman Spectroscopy 為題發表在國際期Analytical Chemistry,城市環境所博士生李弘哲為第一作者,研究員崔麗為通訊作者。該研究得到科技部國家重點研發計劃和國家自然科學基金等的資助。

  文章鏈接 

 

土壤解磷菌拉曼鑒定流程

 

無磷土壤中培養24小時的土壤細菌的單細胞拉曼光譜




(責任編輯:葉瑞優)

附件:

專題推薦

相關新聞


© 1996 - 中國科學院 版權所有 京ICP備05002857號  京公網安備110402500047號  聯系我們

地址:北京市三里河路52號 郵編:100864

网易彩票www.069wy.com www.ji03.com | www.091338.com | www.354119.com | www.020391.com | 010930.com | 168746.com | www.149649.com | www.431328.com | www.693552.com | www.661680.com | www.91629.com | www.961175.com | www.781719.com | www.684291.com | www.z26.in | www.062212.com | www.0808.online | www.902273.com | 2097.pw | www.97tb.cc | 575742.com | www.ybet.cc | www.31ma.com | www.vh19.com | www.34wp.com | www.288248.com | www.eq1.com | www.629125.com | 097536.com | 459905.com | www.7556.in | www.03337.cc | www.m50.site | www.63rs.com | 百姓彩票www.bxcp3.com | www.rf42.com | www.2919.online | www.354436.com | 687093.com | www.379694.com | 162807.com | www.pw3.com | 大玩家彩票www.84499c.com | www.62546.com | www.440358.com | www.008074.com | www.591565.com | 059818.com | 036695.com | 福利彩票www.99677i.com | www.061080.com | 441707.com | www.133911.cc | www.730725.com | www.051.net | www.008600.com | www.233180.com | www.731372.com | www.485187.com | www.270882.com | www.288683.com | www.189.cx | www.707986.com | www.701685.com | www.355835.com | www.9989.pw | 021876.com | www.63cs.com | www.39078.com | www.34or.com | www.902927.com | www.037620.com | www.7606.vip | www.210012.com | www.64on.com | www.139136.com | www.71371.cc | 567319.com | www.76xr.com | www.718.in | 45614.com | www.8380.biz | 902516.com | www.4536.me | www.87653.com | www.134702.com | 903349.com | www.324288.com | www.31971.cc | www.660742.com | www.6429.pw | 183792.com | www.nd20.com | www.388272.com | www.254925.com | www.v75.hk | www.033602.com | www.089256.com | 767666.com | www.4975.biz | www.123388.com | www.500134.com | www.3qk.com | www.630078.com | 897904.com | 428797.com | 99彩票www.577032.com | www.486825.com | www.999057.com | www.080144.com | www.171722.com | 4836.biz | www.kk09.com | www.qy91.com | www.189769.com | 569761.com | 186014.com | www.79ut.com | www.211300.cc | www.529332.com | www.605521.com | www.979367.com | www.036201.com | www.636080.com | www.817454.com | 557912.com | www.822.pw | www.532075.com | www.0979.net | www.075535.com | www.wx52.com | www.573606.com | www.817881.com | www.24269.com | www.106832.com | jk56.cc | 500万彩票www.583792.com | www.47jz.com | www.5908.cm | www.231422.com | www.608337.com | www.kh08.com | www.237040.com | www.992161.com | www.2233.vip | www.160007.com | www.wf4.cc | www.650512.com | www.91ec.com | www.4252.top | www.20402.com | www.23955.com | www.201650.com | www.133184.com | www.750062.com | www.025818.com | www.380321.com | www.809386.com | www.m77.net | www.cl06.com | 184382.com | www.358129.com | www.770453.com | www.632389.com | 5723.biz | www.968286.com | 500彩票网www.65707j.com | www.d78.net | www.247155.com | www.342804.com | 691306.com | www.95je.com | www.3808.top | www.0418.date | www.496870.com | www.680607.com | www.839011.com | www.92320.com | www.604333.cc | 616957.com | www.641814.com | 55sun.com | www.33522.com | www.77640.com | www.872705.com | 680126.com | www.959257.com | www.953286.com | www.43591.com | www.106980.com | 626413.com | www.8685.biz | www.no70.com | www.21359.com | www.28197.cc | www.kz90.com | www.71770.com | 383085.com | uf75.com | www.gb18.com | www.d32.club | 719165.com | www.028573.com | www.441193.com | www.939226.com | www.956083.com | 4565.pw | 全民彩票www.53537.cc | www.233921.com | www.308997.com | 984057.com | www.650524.com | pg73.com | www.202286.com | www.348722.com | www.107041.com | www.918025.com | www.91545.com | 436456.com | www.807079.com | www.896603.com | www.15698.com | www.39877.com | www.673267.com | www.730610.com | www.704737.com | www.607258.com | www.310721.com | www.lk95.com | www.57522.cc | www.92187.com | www.130674.com | www.989089.com | 946979.com | www.317422.com | www.680100.com | www.859201.com | 327931.com | www.bz50.com | www.17913.com | www.ge32.com | www.09916.cc | www.344768.com | www.117754.com | 739554.com | www.706048.com | www.ae08.com | www.79dg.com | www.64gw.com | www.177999.cc | www.789659.com | 639898.com | www.xk74.com | www.cai8177.com | 80660.com | 262444.com | www.qi56.com | 6535.com | www.954062.com | www.708151.com | 259099.com | 450818.com | www.l44.biz | www.06fo.com | www.581.website | www.028324.com | www.106928.com | www.583362.com | www.695283.com | www.707880.com | 537661.com | 159699.com | www.3127.pw | www.9421.cn | www.5762.top | www.8520.vip | www.19972.com | www.014118.com | www.099755.com | www.133105.com | www.333764.com | www.701768.com | www.824300.com | www.05335.com | www.38802.com | www.32982.com | www.3700.top | www.06xr.com | www.vo04.com | www.4gp.cc | www.460073.com | www.563160.com | www.668327.com | www.547706.com | www.269506.com | www.551220.com | 386167.com | 66835.com | 624086.com | 118347.com | 627356.com | 422928.com | 五彩堂www.898341.com | www.37uk.com | www.9518.in | www.025324.com | www.96279.com | www.111242.com | www.123165.com | www.389089.com | www.750506.com | www.3001.top | www.8369.in | www.4243.net | www.874363.com | www.n00.space | www.05db.com | www.5843.cn | www.9557.in | www.077794.com | 592363.com | www.995387.com | 26fs.com | www.980945.com | www.4719.com | www.921816.com | www.fl3.cc | www.wl3.cc | www.ll89.com | www.b13.win | www.36.vip | www.yb62.com | www.js47.cc | www.871.cc | www.19274.com | www.32688.cc | www.202770.com | www.562868.com | www.509151.com | www.897845.com | www.878352.com | www.976473.com | www.924433.com | www.785096.com | www.695971.com | 974213.com | 067971.com | www.co1.com | www.90827.com | www.340518.com | www.760270.com | www.703389.com | www.577591.com | www.506928.com | 268191.com | www.vi29.com | www.56439.cc | www.339421.com | www.791511.com | www.0020.net | www.31hx.com | 090191.com | www.tz88.cc | www.5nt.com | www.w51.club | www.2157.online | www.3312.net | www.39927.cc | www.52992.cc | www.72344.com | www.126655.com | www.374544.com | www.525056.com | www.684809.com | 23725.com | www.998327.com | 286433.com | 949334.com | 870635.com | www.802237.com | www.39269.cc | www.813134.com | www.989058.com | www.947234.com | www.932238.com | 307122.com | www.356147.com | 421644.com | www.62784.com | www.206009.com | www.332454.com | 202622.com | www.23ls.com | www.nj34.com | www.23159.cc | www.286803.com | www.705581.com | www.774841.com | www.573587.com | www.734706.com | www.817952.com | www.11xx.cc | www.238507.com | www.366559.com | www.781624.com | www.226770.com | www.294944.com | 460015.com | 大赢家彩票网www.54400b.com | www.62hk.com | www.770362.com | www.es15.com | www.5982.biz | www.51ct.com | 趣彩网www.qucw9.com | 921761.com | 846012.com | 476821.com | 303985.com | www.838616.com | www.916785.com | www.550349.com | www.j63.me | www.xd42.com | www.87653.com | www.210879.com | www.592315.com | 804000.cc | www.318845.com | www.og97.com | www.198410.com | www.589077.com | www.410247.com | www.91lu.com | www.fs22.com | www.z96.club | www.789061.com | www.737723.com | www.69iq.com | www.63ss.com | www.65qx.com | www.0079.cc | www.26888.com | www.79522.com | www.778333.com | www.963262.com | www.gd75.com | www.xz38.com | www.qi48.com | www.037759.com | www.025914.com | www.082034.com | www.138436.com | www.659063.com | www.990443.com | www.936685.com | www.851881.com | 吉利彩票www.80075h.com | 8号彩票www.81520x.com | www.19783.com | www.725518.com | www.937798.com | www.410563.com | www.696202.com | www.p20.club | www.125062.com | www.482997.com | www.616452.com | 443227.com | www.939900.com | 381910.com | 94ie.com | 761410.com | www.6292.cm | www.6178.cc | www.633.cm | www.7146.vip | www.816109.com | www.153406.com | www.90lh.com | www.995731.com | www.117330.com | 140896.com | www.444764.com | www.fv82.com | www.8401.com | www.4474.cn | www.1115.site | www.71855.cc | www.939651.com | www.2841.cc | www.618080.com | 632331.com | 903776.com | 993465.com | www.552613.com | www.398844.com | 30ec.com | 509853.com | 00039.com | www.m70.com | www.866046.com | www.77kh.com | www.0878.bid | www.67ay.com | www.5976.in | 698014.com | 983493.com | www.509223.com | www.312552.com | www.212142.com | 238039.com | 005780.com | 凤凰彩票www.77803k.com | 919909.com | www.6478.vip | www.890005.com | www.36780.com | www.768912.com | www.813053.com | www.823142.com | www.855780.com | 941100.com | 964374.com | 020894.com | xv18.com | 028937.com | 财神网www.29277f.com | 531285.com | 592763.com | 彩民网www.76520d.com | www.wi22.com | www.99se.cc | www.18295.com | www.29367.cc | www.029914.com | 072116.com | www.53271.com | www.689521.com | www.690324.com | www.759956.com | www.89611.cc | www.63156.com | www.1998.online | www.287329.com | www.593303.com | 570511.com | 377387.com | www.974247.com | www.947436.com | www.828623.com | www.811155.com | www.576434.com | www.576068.com | www.26kl.com | www.387755.com | www.374371.com | www.323918.com | www.055365.com | www.7811vv.com | www.876623.cc | www.74446.com | www.83078.com | 056966.com | www.987298.com | www.062784.com | 百姓彩票www.www.bxcp3333.com | www.566527.com | www.306366.com | www.625653.com | www.666617.com | www.375515.com | www.617151.com | www.940400.com | www.718635.com | cai6111.com | www.279215.com | www.026390.com | www.965791.com | www.921127.com | 3G彩票www.c137.vip | 184177.com | www.72172.com | www.027327.com | www.925375.com | www.96pt.com | www.10jb.com | www.1138.cm | www.18ki.com | 743848.com | www.08981.com | www.95564.cc | www.010616.com | www.5676.net | www.46326.com | www.098679.com | www.080327.com | www.246181.com | www.296911.com | www.383705.com | 35088.com | 104873.com | 793377.com | www.046562.com | www.703899.cc | www.3304.cm | www.407424.com | www.921510.com | www.mn38.com | www.oy75.com | www.1314.pw | www.102894.com | www.69221.cc | www.730483.com | www.917115.com | www.629865.com | cp9848.com | www.mu97.com | www.1117.site | www.20480.com | www.9870.in | www.048.tv | www.072812.com | www.003726.com | www.1869.top | www.ua41.com | www.8181.hk | www.978449.com | www.813247.com | www.563023.com | www.566970.com | www.56ea.com | www.6235.biz | www.71218.com | www.608253.com | 924923.com | www.752718.com | www.653866.com | www.235852.com | 558792.cc | www.505290.com | www.uy97.com | www.274616.com | www.189086.com | www.312262.com | www.229967.com | 811444.com | www.265636.com | 896887.com | www.cp7332.com | 8077789.com | 787677.com | www.10dl.com | www.38pz.cc | www.663356.cc | www.028582.com | 620081.com | 982019.com | 619402.com | yd32.cc | 948419.com | www.290687.com | www.382078.com | 02488b.com | www.973263.com | www.6475.vip | www.027901.com | www.159357.com | www.915789.com | www.835133.com | www.800082.cc | www.640082.com | www.949851.com | www.836519.com | www.900072.cc | www.539142.com | www.158452.com | www.241911.com | www.373726.com | www.731174.com | 051309.com | www.556014.com | www.14688.cc | www.843196.com | www.09255.com | 37791.com | www.196562.com | www.806816.com | www.087177.com | 238970.com | 590839.com | 3308.net | www.880585.com | www.026484.com | www.383150.com | www.693719.com | www.730163.com | www.mq43.com | 751249.com | www.cp9155.com | 574106.cc | 386477.com | www.69op.com | www.cai4766.com | www.169312.com | www.668107.com | www.457247.com | www.0356.pw | www.28511.cc | www.126245.com | www.303663.com | www.966559.com | www.927111.com | 033896.com | www.993302.com | www.807089.com | www.819875.com | www.9916.in | www.93jt.com | www.07nh.com | 973248.com | 4818.biz | www.970264.com | www.859876.com | 27on.com | www.56rk.com | www.rq83.com | www.0rp.com | www.2841.win | www.09uk.com | www.726655.com | www.0365.tv | www.064.pw | www.199753.com | www.225029.com | www.512797.com | www.32vw.com | www.tk30.com | www.12xv.com | www.ch8.cc | www.023690.com | www.329231.com | www.691106.com | www.576519.com | www.379657.com | 714715.com | 911777.com | 177846.com | www.89547.com | www.0555.biz | 873820.com | 964812.com | 677096.com | 657031.com | www.992468.com | www.388949.com | www.085.win | www.772941.com | www.92714.com | www.357338.com | www.59527.cc | www.520261.com | www.677052.com | www.62236.com | 625857.com | www.133049.com | 971570.com | www.468683.com | www.7hf.com | www.89108u.com | 648976.com | 934788.com | 526150.com | www.942858.com | www.78448.com | www.69363.com | www.015443.com | www.911362.com | www.010256.com | www.649478.com | www.js96008.com | 029486.com | 426144.com | www.147121.com | 55彩票www.755910.com | www.89rm.com | www.444734.com | www.260549.com | www.177347.com | 432435.com | 970461.com | 606089.com | www.691871.com | sy10.cc | www.uu90.com | www.qn52.com | www.53yo.com | 625837.com | www.866381.com | www.661029.com | 4554.biz | www.731384.com | www.726058.com | www.618003.com | 170508.com | 543707.com | www.42yw.com | www.154730.com | www.390605.com | www.316201.com | www.539045.com | www.601646.com | www.c26.club | www.921268.com | www.91676.cc | www.974792.com | www.373693.com | www.582690.com | www.417866.com | www.022632.com | www.93xf.com | www.ln33.com | www.239083.com | www.056153.com | 052383.com | 025219.com | 331471.com | 05lt.com | www.675188.com | www.70118.com | 949378.com | www.555.red | 170509.com | www.e52.cn | 顶级娱乐www.047788.com | yk40.com | 270534.com | www.997904.com | 918842.com | www.101988.com | 819938.com | 382030.com | www.826187.com | 685602.com | 353922.com | www.899009.com | www.gf46.com | www.ue94.com | www.gg6.com | www.vo97.com | www.gr86.com | www.181611.com | 022456.com--46651.com | www.1215.xyz | 685301.com | www.134446.com | 317353.com | www.g63.com | www.373719.com | 8338a-www.8205.com | www.982214.com | 482061.com | 60548.cc | www.fi3.cc | www.265887.com | www.805659.com | www.64sz.com | www.360881.com | 338094.com | 500彩票www.247500.com | www.jg34.com | www.2083.com | www.40645.com | cai5777.com | www.929358.com | www.99472.com | 622066.cc | 503207.com | www.086197.com | www.883235.com | www.782992.com | www.77569.com | www.g02.top | 825077.com | www.778893.com | www.8et.com | www.703240.com | www.689703.com | 980914.com | www.923357.com | www.b03.cc | www.68fc.com | 014433.com | 517235.com | www.504585.com | 789460.com | 127604.com | 597675.com | www.228010.com | www.170887.com | www.e20.space | www.314039.com | 500彩票www.979500.com | 5985.biz | www.916634.com | www.kj7.com | www.644.cm | www.02053.com | www.52517.com | www.1963.cc | www.7133.org | www.89ln.com | 170607.com | 558513.com | www.39358.com | www.915382.com | www.521621.com | www.dx71.com | www.833025.com | www.dw31.com | www.rc5.com | 幸运彩票www.852699.com | 562087.com | www.948869.com | www.670846.com | www.830263.com | www.576403.com | www.271044.com | www.703739.com | www.951364.com | www.mo67.com | 798831.com | 4723.biz | www.857787.com | www.997600.cc | www.88tx.cc | www.510166.com | www.83336.com | www.149123.com | www.662247.com | www.128031.com | www.355337.com | www.346213.com | www.651318.com | www.182301.com | www.466016.com | www.27wv.com | www.966802.com | 872680.com | 974826.com | www.34901.com | www.015740.com | 417372.com | 237434.com | 402997.com | 811601.com--163a.com | 692900.com | www.992906.com | www.671164.com | k04.org--k04.org | www.440820.com | www.4641.cn | www.00908.cc | www.875850.com | www.369611.com | www.703269.com | www.861871.com | www.136981.com | www.885577.com | www.uh49.com | www.611685.com | 彩乐乐www.cll168.com | 356241.com | www.am12.cc | www.508949.com | 224987.com | 278792.com | www.788200.com | www.998951.com | www.538800.com | www.w58.org | www.575919.com | www.903633.com | 世彩堂www.5854d.cc | 华夏彩票www.hx1113.com | www.195503.com | 067806.com | 721507.com | www.iq43.com | 366782.com | 22251.com | www.66319.cc | 323822.com | www.951124.com | www.052244.com | www.h22.org | www.323215.com | www.22172.cc | www.3844.in | www.553190.com | www.00223.cc | www.456.in | www.7815.in | www.s60.pw | 大赢家彩票平台www.375769.com | 834787.com | www.sz96.com | ag93.cc | 026751.com | www.977739.com | www.798923.com | www.211133.com | 博乐彩票www.688824.com | 982674.com | 768076.com | 990541.com | 652087.com | www.94xj.com | www.cai7700.com | 077072.com | www.997807.com | 467178.com | 039348.com | www.30uj.com | www.1ii.com | www.89331.com | www.818023.com | www.66717.com | www.17gc.com | 爱购彩票www.3552p.com | 141833.com | 616777.com | www.cx45.com | www.822996.com | www.953391.com | www.658843.com | www.852528.com | www.673920.com | www.121746.com | www.370518.com | www.620825.com | 964986.com | www.365804.cc | www.4649.club | www.751567.com | www.899238.com | www.903816.com | www.653659.com | www.568788.com | www.944345.com | 00857.com | www.2600.wang | www.735565.com | www.410547.com | www.841126.com | 673901.com | 500万彩票www.w935.com | www.637645.com | cai7766.com | gw90.com | www.062696.com | 350576.com | 500万彩票www.06500w.com | www.850934.com | www.18693.cc | www.167523.com | www.445591.com | www.208868.com | www.136551.com | www.033042.com | 590182.com | www.979920.com | 019431.com | www.66631.cc | www.458177.com | www.908829.com | www.040958.com | www.3973.net | www.214493.com | www.40dq.com | www.743205.com | www.5278.site | www.53860.com | www.081051.com | www.810892.com | www.395727.com | www.97528.cc | www.53366.com | www.122770.com | www.133461.com | www.959349.com | 189187.com | www.906959.com | 237894.com | www.61rn.com | 0205.cc | www.072339.com | www.334476.com | www.083903.com | www.ce70.com | www.1503.vip | www.022427.com | www.1766.xyz | www.997657.com | www.878.red | www.3678.biz | www.0188.cc | www.66gc.com | www.604120.com | www.723059.com | www.600141.com | www.64ju.com | www.61137.com | www.537108.com | www.95884.com | www.326213.com | 961480.com | www.978918.com | www.697398.com | www.647767.com | www.199691.com | www.85695.com | www.323865.com | www.8496.vip | www.154784.com | 435879.com | www.469705.com | www.92388.com | www.861281.com | www.168718.com | 181751.com | www.103460.com | www.525360.com | www.130038.com | www.238506.com | 128714.com | 中国福利彩票www.3478p.cc | www.976482.com | www.948711.com | www.100061.com | 幸运彩票www.73166k.com | www.cai2411.com | www.901798.com | www.070046.com | www.678901.cc | 705234.com | 693243.com | www.947305.com | www.567973.com | 933150.com | www.qk93.com | zo49.com | www.950991.com | www.519283.com | www.177875.com | www.381560.com | www.296418.com | www.51862.cc | www.35559.cc | www.8362.loan | 7176.biz | www.039108.com | www.014876.com | 460369.com | www.692495.com | 532.com | 500400.cc | 171825.com | 6383.biz | 4632.biz | www.761346.com | 290028.com | www.gf46.com | www.x11.pw | 237493.com | www.877605.com | www.529816.com | www.444762.com | www.062396.com | 772462.com | www.04895.com | 585399.com | 721389.com | 975451.com | 005890.com | www.76753.com | www.108385.com | www.1523.cc | www.zw87.com | www.937218.com | www.210502.com | www.603211.com | 653662.com | www.753323.com | www.811519.com | 794525.com | 152639.com | 385603.com | www.1917.vip | www.986573.com | www.95pl.com | www.04697.com | www.28734.com | www.k11.com | 028167.com | 897125.com | www.770180.com | 480804.com | www.357897.com | 75796.com | www.578548.com | www.958649.com | 110249.com | www.446770.com | www.55eb.com | www.65er.com | www.535280.com | www.eq40.com | 272591.com | www.900096.com | www.122495.com | www.7223.win | 79qm.com | 5959.com | 03uq.com | www.909713.com | www.978845.com | 857677.com | 696623.com | www.757397.com | www.761609.com | 276585.com | www.co73.com | www.763356.com | www.0578.me | www.766579.com | www.ih71.com | 178797.cc | 9035.biz | www.929913.com | www.983684.com | www.922723.com | www.691787.com | www.535063.com | www.456187.com | www.78486.com | 025604.com | www.552326.com | www.bw07.com | 916075.com | www.454400.com | www.713608.com | www.017972.com | www.cai1833.com | 0892.date | www.192627.com | 984245.com | www.262042.com | www.438506.com | 105994.com | www.747006.com | www.370053.com | www.196212.com | www.1233.pw | www.61qv.com | www.562789.com | www.219496.com | www.89798.cc | www.3950.org | www.074342.com | www.ci38.com | www.945758.com | www.883567.com | www.896488.com | www.3398.net | 500彩票www.54p.com | www.283428.com | www.7vq.com | www.931336.com | www.886391.com | www.47927.com | www.665265.com | www.020510.com | www.303220.com | www.pq22.com | www.751089.com | www.368494.com | www.0823.cc | www.773951.com | www.8465.loan | www.7284.cc | www.752122.com | www.197447.com | www.840898.com | www.547070.com | www.965549.com | www.975074.com | www.538531.com | www.105705.com | www.t37.net | www.gw4.cc | 226984.com | www.700457.com | www.664217.com | www.961917.com | 天玺彩票www.490780.com | 128941.com | 368746.com | 31309.com | www.560124.com | www.392066.com | www.677077.com | www.913738.com | www.507662.com | www.js80.com-84123.com | www.811892.com | www.1399.in | www.37853.cc | www.fa72.com | www.ag47.cc | 428506.com | www.068378.com | www.274626.com | www.352883.com | www.829206.com | www.669595.com | 353407.com--353407.com | www.83bp.com | 680288.com | www.71db.com | 170251.com | www.960910.com | www.732612.com | www.340551.com | www.53361.cc | www.038629.com | www.71fg.com | www.18by.com | 692299.com | www.990873.com | www.855413.com | www.526796.com | 130128.com | www.103640.com | www.5500.biz | www.832663.com | 083711.com | www.755753.com | www.315006.com | www.088.loan | www.808218.com | www.16455.com | 160807.com | www.221028.com | 823547.com | www.nl